Advertisements
Advertisements
प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
Advertisements
उत्तर
In DNA, a palindrome is a sequence of base pairs that reads the same on both strands when the reading orientation is constant.
| (i) | Eco RI: | 5' G A A T T C 3' |
| 3' C T T A A G 5' | ||
| (ii) | Hind III: | 5' A A G C T T 3' |
| 3' T T C G A A 5' | ||
| (iii) | Bam HI: | 5' G G A T C C 3' |
| 3' C CT A G G 5' | ||
| (iv) | Sal I: | 5' G T C G A C 3' |
| 3' C A G C T G 5' | ||
| (v) | Pst I: | 5' C T G C A G 3' |
| 3' G A C G T C 5' |
APPEARS IN
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
How does a restriction nuclease function? Explain
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following bacteria is not a source of restriction endonuclease?
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
What is elution?
State the importance of elution in this process.
What is the precise definition of a palindromic sequence in DNA?
Which of the following was the very first restriction endonuclease to be isolated and characterized?
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
