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प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
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उत्तर
In DNA, a palindrome is a sequence of base pairs that reads the same on both strands when the reading orientation is constant.
| (i) | Eco RI: | 5' G A A T T C 3' |
| 3' C T T A A G 5' | ||
| (ii) | Hind III: | 5' A A G C T T 3' |
| 3' T T C G A A 5' | ||
| (iii) | Bam HI: | 5' G G A T C C 3' |
| 3' C CT A G G 5' | ||
| (iv) | Sal I: | 5' G T C G A C 3' |
| 3' C A G C T G 5' | ||
| (v) | Pst I: | 5' C T G C A G 3' |
| 3' G A C G T C 5' |
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
What is elution?
State the importance of elution in this process.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What is the precise definition of a palindromic sequence in DNA?
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
How are separated DNA bands visualized following agarose gel electrophoresis?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
