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प्रश्न
State the importance of elution in this process.
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उत्तर
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Explain briefly:
Restriction enzymes and DNA
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
How are DNA fragments visualised once they are separated by gel electrophoresis?
How are separated DNA bands visualized following agarose gel electrophoresis?
