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प्रश्न
State the importance of elution in this process.
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उत्तर
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
'Restriction' in restriction enzyme refers to
How does one visualise DNA on an agarose gel?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
