Advertisements
Advertisements
प्रश्न
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Advertisements
उत्तर
If denaturation of double-stranded DNA does not take place, then primers will not be able to anneal to the template, no extension will take place, hence no amplification will occur.
APPEARS IN
संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Describe briefly the following:
Bioreactors
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
Explain briefly:
Chitinase
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
PCR and restriction fragment length polymorphism are the methods for ______.
Gene Amplification using primers can be done by ______.
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
