Advertisements
Advertisements
Question
Which of the following is not required in the preparation of a recombinant DNA molecule?
Options
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
Advertisements
Solution
E.coli
APPEARS IN
RELATED QUESTIONS
Distinguish between plasmid DNA and chromosomal DNA.
Distinguish between RNA and DNA.
What are plasmids?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
What are the restriction endonucleases? Give the rules of their nomenclature.
Describe two vectors less methods of gene transfer used in rDNA technology.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Bt-cotton shows adverse effect on the population of which butterfly?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
From the following identify the methods of electrophoresis.
Which of the following DNA sequence is recognised and cut by EcoR I?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Lytic cycle involves bacteriophage and ____________.
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?

Which of these are most widely used in genetic engineering?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Importance of PCR.
In vitro amplification of DNA segments is called______.
