Advertisements
Advertisements
Question
Which of the following is not required in the preparation of a recombinant DNA molecule?
Options
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
Advertisements
Solution
E.coli
APPEARS IN
RELATED QUESTIONS
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Explain the properties of a good or ideal cloning vector for rDNA technology.
ECoRI is obtained from ______.
Polymerase chain reaction is most useful in ______.
Which of the following is TRUE regarding EcoR I?
The time taken to complete one cycle of PCR is around ______.
Which of the following device is required for PCR?
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The vector Baculovirus is used for gene transfer in ____________.
Name and describe the technique that helps in separation of DNA fragments.
Which molecules are called molecular scissors? Why are they called so?
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Plasmid is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
How gene amplification is done?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
