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Question
State the role of DNA ligase in biotechnology.
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Solution
DNA ligase enzyme is used to join two DNA fragments from their ends.
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RELATED QUESTIONS
Distinguish between RNA and DNA.
Describe two vectors less methods of gene transfer used in rDNA technology.
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i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
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v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
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Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
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5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
In vitro amplification of DNA segments is called______.
Name the source of thermostable DNA polymerase.
