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प्रश्न
State the role of DNA ligase in biotechnology.
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उत्तर
DNA ligase enzyme is used to join two DNA fragments from their ends.
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संबंधित प्रश्न
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Identify the characterization techniques that are based on molecular weights of compounds.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Exonuclease performs the function of ____________.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
Which one of the following enzymes is used to join DNA fragments?
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
