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प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
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उत्तर
Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants for specific proteins. Stanley Cohen and Herbert Boyer accomplished this in 1972.
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संबंधित प्रश्न
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
What is plasmid?
The vector Baculovirus is used for gene transfer in ____________.
Nucleic acid segment tagged with a radioactive molecule is called ______.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Name the enzymes that are called molecular scissors.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
