Advertisements
Advertisements
Question
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Advertisements
Solution
Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants for specific proteins. Stanley Cohen and Herbert Boyer accomplished this in 1972.
APPEARS IN
RELATED QUESTIONS
Alu-I is obtained from which organism?
Which of the following is TRUE regarding EcoR I?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
To be a cloning vector, a plasmid require ______.
How gene amplification is done?
What is EcoRI?
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
