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प्रश्न
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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उत्तर
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Distinguish between RNA and DNA.
Give an account of the Blue-White Method of selection of recombinants.
Name the most commonly used bioreactor and describe its working.
Write a short note on Electrophoresis.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Identify the characterization techniques that are based on molecular weights of compounds.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
Polymerase Chain Reaction is made possible by ____________.
Exonuclease performs the function of ____________.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Name a recombinant vaccine that is currently being used in vaccination programme.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
What is polymerase chain reaction (PCR)?
Write steps involved in gene amplification.
______technique is used for in vitro gene cloning or gene multiplication.
What are the different types of electrophoresis?
How does EcoRI differ from an exonuclease?
