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प्रश्न
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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उत्तर
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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संबंधित प्रश्न
Distinguish between RNA and DNA.
Give an account of the Blue-White Method of selection of recombinants.
How has the development of bioreactor helped in biotechnology?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
ECoRI is obtained from ______.
What is gene cloning? Explain different tools used for it.
Explain types of enzymes used in biotechnology?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the CORRECT sequence of steps in PCR.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
EcoRl & Hind III are the examples of ______
Which molecules are called molecular scissors? Why are they called so?
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
How gene amplification is done?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
