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प्रश्न
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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उत्तर
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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संबंधित प्रश्न
What are plasmids?
Give one significant difference between:
Electroporation and Gene Gun.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Define vector? write any two examples,
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Polymerase Chain Reaction is made possible by ____________.
The gene transfer between unrelated plants is brought about by ____________.
Exonuclease performs the function of ____________.
What is meant by gene cloning?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
The PCR technique is used for ______.
Describe three steps involved in mechanism of PCR.
______technique is used for in vitro gene cloning or gene multiplication.
How does EcoRI differ from an exonuclease?
Which amino acid is coded by the initiation codon?
What is the relationship between alien DNA and the origin of replication in the concept of cloning?
