Advertisements
Advertisements
Question
Annealing step of PCR operates at ______ °C.
Options
90-98
40-60
70-75
100-120
Advertisements
Solution
Annealing step of PCR operates at 40-60 °C.
APPEARS IN
RELATED QUESTIONS
Expand the following abbreviation: BAC
What is the cell that receives a recombinant gene called?
Explain the properties of a good or ideal cloning vector for rDNA technology.
Which of the following is TRUE regarding EcoR I?
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Polymerase Chain Reaction is made possible by ____________.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
Importance of PCR.
