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Question
Describe the role of CaCl2 in the preparation of competent cells?
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Solution
CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bactrial cells. The divalent Ca+2 ions supposedly create transient pores on the bactrial cell wall by which the entry of foreign DNA is facilitated into the bactrial cells.
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RELATED QUESTIONS
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Choose the correct answer from the options given below:
