Advertisements
Advertisements
Question
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Advertisements
Solution
T-DNA is the only essential part required to make Ti plasmid a cloning vector. The plasmid is disarmed by deleting the tumour-inducing genes in the plasmid so that it becomes an effective cloning vector and remove it harmful effect.
APPEARS IN
RELATED QUESTIONS
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
The genetic material of virus includes:
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following is correctly matched?
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Describe the role of CaCl2 in the preparation of competent cells?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
What is the basic biological nature of plasmids when used as cloning vectors?
Which of the following describes the function of a selectable marker in a cloning vector?
What is the primary role of a cloning site in a vector?
In blue–white screening, what do the white colonies indicate?
Which of the following is a group of vectors used for gene transfer into animals?
