Advertisements
Advertisements
Question
The figure below shows the structure of a plasmid.

A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
Options
Medium with Tetracycline Medium with Ampicillin Growth No growth Medium with Tetracycline Medium with Ampicillin No growth Growth Medium with Tetracycline Medium with Ampicillin No growth No growth Medium with Tetracycline Medium with Ampicillin Growth Growth
Advertisements
Solution
| Medium with Tetracycline | Medium with Ampicillin |
| No growth | Growth |
APPEARS IN
RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
A clone is a ______.
Which of the following is not a genetic vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of CaCl2 in the preparation of competent cells?
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
