Advertisements
Advertisements
Question
The figure below shows the structure of a plasmid.

A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
Options
Medium with Tetracycline Medium with Ampicillin Growth No growth Medium with Tetracycline Medium with Ampicillin No growth Growth Medium with Tetracycline Medium with Ampicillin No growth No growth Medium with Tetracycline Medium with Ampicillin Growth Growth
Advertisements
Solution
| Medium with Tetracycline | Medium with Ampicillin |
| No growth | Growth |
APPEARS IN
RELATED QUESTIONS
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Write the role of ‘restriction sites’ in the cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
Plasmids are suitable vectors for gene cloning because these ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
