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प्रश्न
What is polymerase chain reaction (PCR)?
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उत्तर १
Polymerase chain reaction (PCR) is another device used for gene cloning or gene multiplication in vitro.
उत्तर २
Polymerase Chain Reaction (PCR) is an in vitro technique used to generate millions of copies of a specific DNA segment rapidly. PCR involves three main steps repeated in cycles: denaturation (heating DNA to separate strands), annealing (binding of primers to target DNA), and extension (DNA polymerase synthesizes new DNA). The enzyme used is Taq polymerase, which is heat-stable and derived from Thermus aquaticus. PCR amplifies DNA to make numerous copies from a small initial sample, facilitating genetic research and diagnostics.
संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
What does PCR stand for? Describe the different steps of PCR
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
The biological scissor is ______.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Explain the properties of a good or ideal cloning vector for rDNA technology.
The enzyme restriction endonuclease ______.
Ti plasmid being used for introducing genes in plants obtained from ______.
What is plasmid?
What is Palindromic sequence?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
The vector Baculovirus is used for gene transfer in ____________.
Lytic cycle involves bacteriophage and ____________.
Which molecules are called molecular scissors? Why are they called so?
Which of the following statements is correct with respect to cellular respiration?
What is meant by gene cloning?
Which vector is mostly used in rDNA technology in plants?
Which one of the following enzymes is used to join DNA fragments?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
