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प्रश्न
What is polymerase chain reaction (PCR)?
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उत्तर १
Polymerase chain reaction (PCR) is another device used for gene cloning or gene multiplication in vitro.
उत्तर २
Polymerase Chain Reaction (PCR) is an in vitro technique used to generate millions of copies of a specific DNA segment rapidly. PCR involves three main steps repeated in cycles: denaturation (heating DNA to separate strands), annealing (binding of primers to target DNA), and extension (DNA polymerase synthesizes new DNA). The enzyme used is Taq polymerase, which is heat-stable and derived from Thermus aquaticus. PCR amplifies DNA to make numerous copies from a small initial sample, facilitating genetic research and diagnostics.
संबंधित प्रश्न
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
What is plasmid?
What is the role of Taq-polymerase in PCR technology?
Explain types of enzymes used in biotechnology?
Identify the CORRECT sequence of steps in PCR.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
______ is a Type I restriction enzyme.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Which one is a true statement regarding DNA polymerase used in PCR?
The PCR technique is used for ______.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Plasmid is ______.
Write steps involved in gene amplification.
The restriction enzymes used as molecular scissors are type of ______.
How does the concept of cloning relate to the multiplication of genetic material in biotechnology?
