Advertisements
Advertisements
प्रश्न
How does restriction endonuclease function?
Advertisements
उत्तर
Each restriction endonuclease finds its specific palindromic nucleotide sequences in the DNA and cuts the DNA at these specific sites. It does so by binding to the DNA at these sites and cutting both the strands at specific points in their sugar-phosphate backbones.
APPEARS IN
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
While isolating DNA from bacteria, which of the following enzymes is not required?
Would you choose an exonuclease while producing a recombinant DNA molecule?
What is elution?
State the importance of elution in this process.
Identify the activity of endonuclease and exonuclease in the given image.
