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प्रश्न
How does restriction endonuclease function?
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उत्तर
Each restriction endonuclease finds its specific palindromic nucleotide sequences in the DNA and cuts the DNA at these specific sites. It does so by binding to the DNA at these sites and cutting both the strands at specific points in their sugar-phosphate backbones.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Which of the following bacteria is not a source of restriction endonuclease?
Would you choose an exonuclease while producing a recombinant DNA molecule?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
