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प्रश्न
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
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उत्तर
If the restriction enzymes have more than one recognition site in a vector then the vector itself will get fragmented on treatment with the restriction enzyme.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
How does a restriction nuclease function? Explain
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Distinguish between exonuclease and endonuclease.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
DNA fragments separate according to size through?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following bacteria is not a source of restriction endonuclease?
Which of the following statements does not hold true for restriction enzyme?
Would you choose an exonuclease while producing a recombinant DNA molecule?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
State the importance of elution in this process.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
How are separated DNA bands visualized following agarose gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
