हिंदी
कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?

Advertisements
Advertisements

प्रश्न

Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?

विकल्प

  • DNA can be seen in visible light

  • DNA can be seen without staining in visible light

  • Ethidium bromide stained DNA can be seen in visible light

  • Ethidium bromide stained DNA can be seen under exposure to UV light

MCQ
Advertisements

उत्तर

Ethidium bromide stained DNA can be seen under exposure to UV light

Explanation:

DNA molecules separated by agarose gel electrophoresis are not visible in normal visible light and require staining. Ethidium bromide binds to DNA and fluoresces under UV light, allowing visualization of DNA bands as bright orange coloured bands. This method is widely used in laboratories for DNA analysis after gel electrophoresis.

shaalaa.com
  क्या इस प्रश्न या उत्तर में कोई त्रुटि है?
अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७५]

APPEARS IN

एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 4. | पृष्ठ ७५
नूतन Biology [English] Class 12 ISC
अध्याय 11 Principles and Processes of Biotechnology
Test Your Progress | Q 1. 42. | पृष्ठ ५३१

संबंधित प्रश्न

How are 'sticky ends' formed on a DNA strand? Why are they so called? 


Mention the difference in the mode of action of exonuclease and endonuclease.


Suggest a technique to a researcher who needs to separate fragments of DNA.


How does a restriction nuclease function? Explain


Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease


Explain briefly:

Restriction enzymes and DNA


Distinguish between exonuclease and endonuclease.


Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.


Give a reason why :
Single cloning site is preferred in a vector.


Which of the following radioisotope is not suitable for DNA labeling based studies?


'Restriction' in restriction enzyme refers to


DNA fragments separate according to size through?


A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______


Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?


'Restriction' in Restriction enzyme refers to ______.


While isolating DNA from bacteria, which of the following enzymes is not required?


The role of DNA ligase in the construction of a recombinant DNA molecule is ______.


Which of the following statements does not hold true for restriction enzyme?


Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?


Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing 

  1. Anode end
  2. smallest/lightest DNA strand in the matrix
  3. Agarose gel


What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.


State the principle involved in separation of DNA fragments using gel electrophoresis.


What is the precise definition of a palindromic sequence in DNA?


Which of the following was the very first restriction endonuclease to be isolated and characterized?


Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?


During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?


What is the origin of agarose, the matrix most commonly used in gel electrophoresis?


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×