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प्रश्न
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
विकल्प
DNA can be seen in visible light
DNA can be seen without staining in visible light
Ethidium bromide stained DNA can be seen in visible light
Ethidium bromide stained DNA can be seen under exposure to UV light
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उत्तर
Ethidium bromide stained DNA can be seen under exposure to UV light
Explanation:
DNA molecules separated by agarose gel electrophoresis are not visible in normal visible light and require staining. Ethidium bromide binds to DNA and fluoresces under UV light, allowing visualization of DNA bands as bright orange coloured bands. This method is widely used in laboratories for DNA analysis after gel electrophoresis.
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संबंधित प्रश्न
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DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
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A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
'EcoRI' has played a very significant role in rDNA technology.
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- Write the recognition site and the cleavage sites of this restriction endonuclease.
State the principle involved in separation of DNA fragments using gel electrophoresis.
How are DNA fragments visualised once they are separated by gel electrophoresis?
