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प्रश्न
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
विकल्प
DNA can be seen in visible light
DNA can be seen without staining in visible light
Ethidium bromide stained DNA can be seen in visible light
Ethidium bromide stained DNA can be seen under exposure to UV light
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उत्तर
Ethidium bromide stained DNA can be seen under exposure to UV light
Explanation:
DNA molecules separated by agarose gel electrophoresis are not visible in normal visible light and require staining. Ethidium bromide binds to DNA and fluoresces under UV light, allowing visualization of DNA bands as bright orange coloured bands. This method is widely used in laboratories for DNA analysis after gel electrophoresis.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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Restriction Enzymes
Answer the following question.
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Applying Chargaff’s rule, what conclusion can be drawn?
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
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DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
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Would you choose an exonuclease while producing a recombinant DNA molecule?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
