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प्रश्न
What does ‘competent’ refer to in competent cells used in transformation experiments?
What does ‘competent’ refer to in competent cells used in transformation?
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उत्तर
Competent means bacterial cells, on treatment with CaCl₂, are made capable of taking up foreign DNA.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The genetic material of virus includes:
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A clone is a ______.
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
