Advertisements
Advertisements
प्रश्न
What does ‘competent’ refer to in competent cells used in transformation experiments?
What does ‘competent’ refer to in competent cells used in transformation?
Advertisements
उत्तर
Competent means bacterial cells, on treatment with CaCl₂, are made capable of taking up foreign DNA.
APPEARS IN
संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
Which of the following is not a cloning vector?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
What is the basic biological nature of plasmids when used as cloning vectors?
In blue–white screening, what do the white colonies indicate?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
Which of the following is a group of vectors used for gene transfer into animals?
