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प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
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उत्तर
Agrobacterium is a bacterium that infects plant tissues by transferring its plasmid T-DNA to the plant genome. This property of Agrobacterium is exploited in the biotechnology industry.
The desired gene that has to be to transferred to a particular plant is inserted in a plasmid T-DNA of Agrobacterium. Then, this engineered Agrobacterium is allowed to infect that particular plant, so that it can transfer the desired gene to the plant genome through its T-DNA. In this way, the desired changes are made in the plant.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
