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प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
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उत्तर
Agrobacterium is a bacterium that infects plant tissues by transferring its plasmid T-DNA to the plant genome. This property of Agrobacterium is exploited in the biotechnology industry.
The desired gene that has to be to transferred to a particular plant is inserted in a plasmid T-DNA of Agrobacterium. Then, this engineered Agrobacterium is allowed to infect that particular plant, so that it can transfer the desired gene to the plant genome through its T-DNA. In this way, the desired changes are made in the plant.
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संबंधित प्रश्न
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
The genetic material of virus includes:
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
