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प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
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उत्तर
Agrobacterium is a bacterium that infects plant tissues by transferring its plasmid T-DNA to the plant genome. This property of Agrobacterium is exploited in the biotechnology industry.
The desired gene that has to be to transferred to a particular plant is inserted in a plasmid T-DNA of Agrobacterium. Then, this engineered Agrobacterium is allowed to infect that particular plant, so that it can transfer the desired gene to the plant genome through its T-DNA. In this way, the desired changes are made in the plant.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Which of the following is correctly matched?
What does ‘competent’ refer to in competent cells used in transformation experiments?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
Which of the following describes the function of a selectable marker in a cloning vector?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
What is the primary role of a cloning site in a vector?
