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प्रश्न
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
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उत्तर
The selective marker is used in the selection of recombinants on the basis of the ability to produce colour in the presence of a chromogenic substrate. β-galactosidase is an enzyme that converts galactose into lactose. In this, a recombinant DNA is inserted within the coding sequence of enzyme, β-galactosidase, which results in inactivation of an enzyme referred to as "insertional inactivation".
The coding sequence for the enzyme β-galactosidase is preferred over antibiotic resistance genes because recombinants can be easily visualized.
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संबंधित प्रश्न
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
What is ‘Ori’ ? State its importance during cloning of a vector.
The genetic material of virus includes:
Which of the following is not a genetic vector?
Which of the following is not a cloning vector?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
What is the basic biological nature of plasmids when used as cloning vectors?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
