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प्रश्न
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
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उत्तर
The selective marker is used in the selection of recombinants on the basis of the ability to produce colour in the presence of a chromogenic substrate. β-galactosidase is an enzyme that converts galactose into lactose. In this, a recombinant DNA is inserted within the coding sequence of enzyme, β-galactosidase, which results in inactivation of an enzyme referred to as "insertional inactivation".
The coding sequence for the enzyme β-galactosidase is preferred over antibiotic resistance genes because recombinants can be easily visualized.
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संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
A clone is a ______.
Which of the following is not a cloning vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
What does ‘competent’ refer to in competent cells used in transformation experiments?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
