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प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
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उत्तर १
In the cloning vector pBR322, ampicillin and tetracycline resistance genes serve as selectable markers. They help in the selection of transformed cells from nontransformed cells. They also help distinguish recombinant cells from nonrecombinant cells.
उत्तर २
The selectable markers in the cloning vector pBR322 are the genes encoding resistance to antibiotics, specifically ampicillin and tetracycline. These markers play a crucial role in identifying and selecting transformants. When a foreign DNA is inserted into the vector, it disrupts one of the antibiotic resistance genes, often the tetracycline resistance gene. This results in transformants that are resistant to ampicillin but sensitive to tetracycline, allowing selection of recombinant cells. The markers enable growth of only those bacteria that have taken up the plasmid and help distinguish recombinants from non-recombinants through antibiotic sensitivity patterns.
संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
Which of the following is not a cloning vector?
