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प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
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उत्तर १
In the cloning vector pBR322, ampicillin and tetracycline resistance genes serve as selectable markers. They help in the selection of transformed cells from nontransformed cells. They also help distinguish recombinant cells from nonrecombinant cells.
उत्तर २
The selectable markers in the cloning vector pBR322 are the genes encoding resistance to antibiotics, specifically ampicillin and tetracycline. These markers play a crucial role in identifying and selecting transformants. When a foreign DNA is inserted into the vector, it disrupts one of the antibiotic resistance genes, often the tetracycline resistance gene. This results in transformants that are resistant to ampicillin but sensitive to tetracycline, allowing selection of recombinant cells. The markers enable growth of only those bacteria that have taken up the plasmid and help distinguish recombinants from non-recombinants through antibiotic sensitivity patterns.
संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
What is ‘Ori’ ? State its importance during cloning of a vector.
A clone is a ______.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
Name the regions marked A, B and C.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
