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प्रश्न
What is ‘Ori’ ? State its importance during cloning of a vector.
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उत्तर
Origin of Replication (Ori):- This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.
Importance of Ori:-
(i) It is responsible for controlling the copy number & the linked DNA.
(ii) In cloning if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following is correctly matched?
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a cloning vector?
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
Which of the following is not a cloning vector?
What is the primary role of a cloning site in a vector?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
