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प्रश्न
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
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उत्तर
In a gene, cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector (the step would not be affected) and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant/plasmid DNA, in the absence of a selectable marker, it will be difficult to distinguish between transformants and non-transformants, because role of the selectable marker is in the selection of transformants.
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संबंधित प्रश्न
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
The genetic material of virus includes:
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A clone is a ______.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a cloning vector?
Name the regions marked A, B and C.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
Which of the following enzyme(s) are NOT essential for gene cloning?
- Restriction enzymes
- DNA ligase
- DNA mutase
- DNA recombinase
- DNA polymerase
Choose the correct answer from the options given below:
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
What is the primary function of a vector in recombinant DNA technology?
What is the basic biological nature of plasmids when used as cloning vectors?
Which of the following describes the function of a selectable marker in a cloning vector?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
In blue–white screening, what do the white colonies indicate?
Which of the following is a group of vectors used for gene transfer into animals?
