Question

What is the function of DNA ligase in the creation of recombinant DNA?

Options

• DNA ligase removes undesirable genes from DNA sequences

• DNA ligase cuts DNA at specific restriction sites

• DNA ligase acts on cut DNA molecules and joins their ends

• DNA ligase replicates DNA in the host organism

Answer 1

DNA ligase acts on cut DNA molecules and joins their ends. In the creation of recombinant DNA, DNA ligase joins the antibiotic resistance gene to the plasmid vector, creating the new combination of circular autonomously replicating DNA formed in vitro that is called recombinant DNA.