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प्रश्न
Do eukaryotic cells have restriction endonucleases? Justify your answer.
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उत्तर
Yes, restriction endonucleases are found in eukaryotic cells. Restriction endonuclease acts by examining the length of the DNA sequence. When it finds its specific recognition sequence, it binds to the DNA and cuts both strands of the double helix at specific points in the sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in DNA.
संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain briefly:
Restriction enzymes and DNA
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Molecular scissors, which cut DNA at specific site is ______.
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the following bacteria is not a source of restriction endonuclease?
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
State the importance of elution in this process.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
