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प्रश्न
Define vector? write any two examples,
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उत्तर
- Cloning vectors are DNA molecules that carry a foreign DNA segment and replicate inside the host cell.
- Following are the different types of vectors used in rDNA technology: Vectors may be plasmids, bacteriophages (M13, lambda virus).
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संबंधित प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
The technique which involves addition or deletion of genes is ______.
ECoRI is obtained from ______.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
Which of the following viruses has the capacity to produce lysozymes?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Write steps involved in gene amplification.
