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प्रश्न
Define vector? write any two examples,
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उत्तर
- Cloning vectors are DNA molecules that carry a foreign DNA segment and replicate inside the host cell.
- Following are the different types of vectors used in rDNA technology: Vectors may be plasmids, bacteriophages (M13, lambda virus).
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संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
Describe two vectors less methods of gene transfer used in rDNA technology.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
From the following which one is NOT a feature of the plasmids?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Name the plant disease caused by Agrobacterium tumefaciens.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The DNA polymerase enzyme used in PCR is obtained from______.
