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प्रश्न
Define vector? write any two examples,
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उत्तर
- Cloning vectors are DNA molecules that carry a foreign DNA segment and replicate inside the host cell.
- Following are the different types of vectors used in rDNA technology: Vectors may be plasmids, bacteriophages (M13, lambda virus).
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संबंधित प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Polymerase Chain Reaction is made possible by ____________.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Expand the following acronym:
BAC
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
