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प्रश्न
Define vector? write any two examples,
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उत्तर
- Cloning vectors are DNA molecules that carry a foreign DNA segment and replicate inside the host cell.
- Following are the different types of vectors used in rDNA technology: Vectors may be plasmids, bacteriophages (M13, lambda virus).
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संबंधित प्रश्न
The biological scissor is ______.
Why are bio-fertilizers preferred over chemical fertilizers?
The technique which involves addition or deletion of genes is ______.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Restriction enzymes were discovered by ______.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write steps involved in gene amplification.
How does EcoRI differ from an exonuclease?
