Advertisements
Advertisements
Questions
What would be the molar concentration of human DNA in a human cell?
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
Advertisements
Solution
Human diploid cell contains about 6 × 10–12 g DNA.
Human genome size ≈ 6.4 × 109 base pairs
Average mass of 1 base pair ≈ 660 g mol–1
Molar mass of DNA = 6.4 × 109 × 660
= 4.2 × 1012 g mol–1
Moles of DNA per cell = `(6xx10^-12)/(4.2xx10^12)`
= 1.4 × 10–24 mol
Taking average cell volume = 10–12 L
Concentration = `(1.4xx10^-24)/10^-12`
= 1.4 × 10–12 M
APPEARS IN
RELATED QUESTIONS
State the role of DNA ligase in biotechnology.
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Which of the following is TRUE regarding EcoR I?
Identify the CORRECT sequence of steps in PCR.
Which of the following device is required for PCR?
Which of the following is an INCORRECT match?
What is true for plasmid?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Restriction enzymes were discovered by ______.
Which vector is mostly used in rDNA technology in plants?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
Describe three steps involved in mechanism of PCR.
The polymerase chain reaction is a powerful technique to ______.
Write a short note on vectors.
Explain the mechanism of PCR.
Which one of the following enzymes is used to join DNA fragments?
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
