Advertisements
Advertisements
Question
What is Recognition sequence? Explain in brief.
Advertisements
Solution
- The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry is known as recognition sequence.
- When the sequence is read in opposite direction (3’ to 5’ or 5’ to 3’) it is identical/ same. For e.g. consider the particular sequence recognized by the enzyme EcoRI. 3’ -______ CTTAAG______ 5’ 5’______ GAATTC ______ 3’
- Restriction enzymes either cut straight across the DNA in the region of palindrome to give blunt ends or cuts producing short, single stranded projections at each end of DNA to produce, cohesive or sticky ends or staggered ends.
RELATED QUESTIONS
Distinguish between plasmid DNA and chromosomal DNA.
In vitro amplification of DNA or RNA segment is known as ___________.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is an INCORRECT match?
Which of the following DNA sequence is recognised and cut by EcoR I?
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
The vector Baculovirus is used for gene transfer in ____________.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Which vector is mostly used in rDNA technology in plants?
