Question

Which of the following DNA sequence is recognised and cut by EcoR I?

Options

• 3'ACGAATTCAT5'

  5'TGCITAAGTA3 '

• 5'ACGAAITCAT3 '

  3 'TGCTT AAGT A5'

• 5'TACTTAAGCA3'

  3'ATGAATTCGT5 '

• 5'TGCTTAAGTA3 '

  3 'ACGAA TTCAT5'

Answer 1

5'ACGAAITCAT3 '

3 'TGCTT AAGT A5'

Explanation:

EcoR I is an E. coli-derived endonuclease enzyme. EcoR I recognise the following sequence: 5'GAATTC3' \s3'CTTAAG5'