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प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
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उत्तर
Taq polymerase is an enzyme obtained from Thermus aquaticus which is a bacterium that normally lives in hot water springs. It is used in amplifying small quantities of short segments of DNA by polymerase chain reaction.
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संबंधित प्रश्न
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Define biotechnology? Which are the basic principles and process of biotechnology?
The time taken to complete one cycle of PCR is around ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Expand YAC used in the field of Biotechnology.
How many sets of primers are required in each cycle of PCR?
Give a reason for the following:
DNA cannot enter directly into the host cell.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
