Question

Which of the following enzyme(s) are NOT essential for gene cloning?

1. Restriction enzymes
2. DNA ligase
3. DNA mutase
4. DNA recombinase
5. DNA polymerase

Choose the correct answer from the options given below:

विकल्प

• C and D only

• A and B only

• D and E only

• B and C only

Answer 1

C and D only

Explanation:

In gene cloning, the essential enzymes are:

• Restriction enzymes: Cut DNA at specific sites.
• DNA ligase: Joins DNA fragments.
• DNA polymerase: Amplifies DNA sequences.

DNA mutase and DNA recombinase are not essential for the core steps of cloning:

Unlike conventional cloning, DNA mutase is engaged in mutation processes. Although it helps with recombination, DNA recombinase is not necessary for conventional gene cloning procedures. Therefore, options D (DNA recombinase) and C (DNA mutase) are not necessary.