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Question
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
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Solution
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RELATED QUESTIONS
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Define vector? write any two examples,
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
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ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
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| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
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Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
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5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
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- Ability to multiply and express outside the host in a bioreactor.
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Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
The DNA polymerase enzyme used in PCR is obtained from______.
How gene amplification is done?
How does EcoRI differ from an exonuclease?
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
