Advertisements
Advertisements
Question
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Advertisements
Solution
- Restriction enzymes cut the DNA at a specific recognition site and produce either sticky ends or blunt ends.
- For example, if we use the restriction enzyme EcoRI to cut vector DNA, then it will produce vector DNA having sticky ends, and if we use the Hind II restriction enzyme to cut donor DNA, then it will produce donor DNA with blunt ends.
- A vector DNA to get ligated to the desired gene, they both must possess similar kinds of ends, i.e., both should have either blunt ends or sticky ends.
- If two separate restriction enzymes are used to cut vector and donor DNA, then they will fail to form complementary base pairing, and chimeric DNA will not be formed.
RELATED QUESTIONS
Recombinant protein .................. is used to dissolve blood clots present in the body.
Which of the following term represents pairing of primers to the ssDNA segment during PCR?
Study the following statements regarding proteins that are being produced by transgenic crop plants and select the correct option.
i. Human testosterone hormone is produced by gene inserted into the chloroplast DNA of tobacco plants.
ii. Protein antigens to be used in vaccines for e.g. Patient-specific antilymphoma (a cancer) vaccines.
iii. In transgenic plants, Humanized antibodies can be produced against HIV, Respiratory syncytial virus (RSV), Herpes simplex virus (HSY).
Which of the following is used for assisting the transfer of rDNA into a bacterial cell?
Which of the following is used for the isolation and purification of the desired product produced by transformed cells?
Identify the process by which competent host can take up chimeric DNA.
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
Plasmid DNA containing foreign DNA is called______.
Which one of the following is NOT true regarding restriction endonucleases?
They are ______.
