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प्रश्न
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
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उत्तर
- Restriction enzymes cut the DNA at a specific recognition site and produce either sticky ends or blunt ends.
- For example, if we use the restriction enzyme EcoRI to cut vector DNA, then it will produce vector DNA having sticky ends, and if we use the Hind II restriction enzyme to cut donor DNA, then it will produce donor DNA with blunt ends.
- A vector DNA to get ligated to the desired gene, they both must possess similar kinds of ends, i.e., both should have either blunt ends or sticky ends.
- If two separate restriction enzymes are used to cut vector and donor DNA, then they will fail to form complementary base pairing, and chimeric DNA will not be formed.
संबंधित प्रश्न
Give the role of Ca++ ions in the transfer of the recombinant vector into the bacterial host cells.
Explain the steps in process of rDNA technology with suitable diagrams.
Study the following statements regarding proteins that are being produced by transgenic crop plants and select the correct option.
i. Human testosterone hormone is produced by gene inserted into the chloroplast DNA of tobacco plants.
ii. Protein antigens to be used in vaccines for e.g. Patient-specific antilymphoma (a cancer) vaccines.
iii. In transgenic plants, Humanized antibodies can be produced against HIV, Respiratory syncytial virus (RSV), Herpes simplex virus (HSY).
Which of the following is/are the CORRECT step/s involved in construction of a recombinant DNA?
Identify the techniques that can be used to transfer foreign DNA directly into the cell or protoplast without the use of plasmids.
i. Biolistic method
ii. Ultrasonification
iii. Shot gun
iv. Lipofection
v. Microinjection
vi. Electroporation
During gene cloning, the enzyme ______ is used to join the gene of interest with the plasmid vector.
Identify the CORRECT statements regarding edible vaccines.
Lambda phage which is commonly used as a cloning vector in rDNA technology usually infects ____________.
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
