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प्रश्न
Give the role of Ca++ ions in the transfer of the recombinant vector into the bacterial host cells.
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उत्तर
Ca++ ions assist the transfer of the recombinant vector into a bacterial host cell.
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संबंधित प्रश्न
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Explain the steps in process of rDNA technology with suitable diagrams.
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
Which of the following is used for the isolation and purification of the desired product produced by transformed cells?
Identify the techniques that can be used to transfer foreign DNA directly into the cell or protoplast without the use of plasmids.
i. Biolistic method
ii. Ultrasonification
iii. Shot gun
iv. Lipofection
v. Microinjection
vi. Electroporation
Identify the process by which competent host can take up chimeric DNA.
Lambda phage which is commonly used as a cloning vector in rDNA technology usually infects ____________.
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Define genomic library.
Name the following:
Human protein produced by rDNA technology to treat cancer.
