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Question
Give the role of Ca++ ions in the transfer of the recombinant vector into the bacterial host cells.
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Solution
Ca++ ions assist the transfer of the recombinant vector into a bacterial host cell.
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RELATED QUESTIONS
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Explain the steps in process of rDNA technology with suitable diagrams.
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
Study the following statements regarding proteins that are being produced by transgenic crop plants and select the correct option.
i. Human testosterone hormone is produced by gene inserted into the chloroplast DNA of tobacco plants.
ii. Protein antigens to be used in vaccines for e.g. Patient-specific antilymphoma (a cancer) vaccines.
iii. In transgenic plants, Humanized antibodies can be produced against HIV, Respiratory syncytial virus (RSV), Herpes simplex virus (HSY).
A ______ refers to the competent host cell that has taken up chimeric DNA.
Identify the techniques that can be used to transfer foreign DNA directly into the cell or protoplast without the use of plasmids.
i. Biolistic method
ii. Ultrasonification
iii. Shot gun
iv. Lipofection
v. Microinjection
vi. Electroporation
During gene cloning, the enzyme ______ is used to join the gene of interest with the plasmid vector.
Identify the process by which competent host can take up chimeric DNA.
Define genomic library.
Which one of the following is NOT true regarding restriction endonucleases?
They are ______.
