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प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
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उत्तर
The correct sequence of the steps involved in biotechnological reaction are
Genomic DNA template
↓
Denaturation of ds-DNA
↓
Nucleotides provided
↓
Primers
↓
Thermostable DNA polymerase (from Thermus aquaticus )
↓
Enzyme DNA polymerase
↓
Chemically synthesised oligonucleotides
↓
Complementary region of DNA
↓
In vitro synthesis of copies of DNA of interest
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संबंधित प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
____________ cuts the DNA within the specific positions.
Which one is a true statement regarding DNA polymerase used in PCR?
What is true for plasmid?
The PCR technique is used for ______.
Modern biotechnology is based on two core techniques. Which of the following correctly identifies both of these techniques?
Genetic engineering is also known as:
