Advertisements
Advertisements
Question
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Advertisements
Solution
The correct sequence of the steps involved in biotechnological reaction are
Genomic DNA template
↓
Denaturation of ds-DNA
↓
Nucleotides provided
↓
Primers
↓
Thermostable DNA polymerase (from Thermus aquaticus )
↓
Enzyme DNA polymerase
↓
Chemically synthesised oligonucleotides
↓
Complementary region of DNA
↓
In vitro synthesis of copies of DNA of interest
APPEARS IN
RELATED QUESTIONS
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
Give an account of the Blue-White Method of selection of recombinants.
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
ECoRI is obtained from ______.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
From the following identify the methods of electrophoresis.
Annealing step of PCR operates at ______ °C.
Name two restriction enzymes used in PCR.
______technique is used for in vitro gene cloning or gene multiplication.
The three basic steps in genetic modification are:
