हिंदी

Enzymes used in DNA Replication

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Estimated time: 2 minutes
CBSE: Class 12
CISCE: Class 12

Key Points: Enzymes Used in DNA Replication

Enzyme Function
Primase Synthesises short RNA primers (A, U, G, C) providing a free 3′-OH end
Helicase Unwinds the DNA double helix
Topoisomerase Relieves supercoiling; cuts and rejoins one strand ahead of the fork
DNA Polymerase Adds deoxyribonucleotides to the 3′-OH end; synthesises only in 5′ → 3′ direction
DNA Ligase Joins DNA fragments by forming phosphodiester bonds between 3′-OH and 5′-phosphate ends
Repair Enzymes (Nucleases) Correct replication errors as part of the polymerase complex
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