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Induction of Gene Mutation

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Estimated time: 3 minutes
CISCE: Class 12

Key Points: Induction of Gene Mutation

Mutagen Category Mode of Action / Effect
5-Bromouracil (BU) Chemical Replaces thymine (pairs with guanine).
2-Aminopurine (AP) Replaces adenine (pairs with cytosine).
Nitrous acid Deamination and strand crossing over.
Hydroxylamine Hydroxylation of cytosine.
Ethylmethane sulphonate Alkylation of purines, transitions.
Acridine dyes (e.g., orange) Frameshift mutations by intercalation.
Colchicine Induces polyploidy (inhibits spindle formation).
Ultraviolet (UV) rays Radiation (Non-ionizing) Forms pyrimidine dimers, causing repair errors.
X-rays & Ionizing Radiation Radiation (Ionizing) Breakage of single- and double-stranded DNA.
High Temperature Physical Disturbs genes, causing mutation.
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