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Overview of Biotechnology - Principles and Processes

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CBSE: Class 12

Key Points: Principles of Biotechnology

  • Biotechnology is based mainly on genetic engineering and bioprocess engineering.
  • Genetic engineering involves altering DNA/RNA and introducing desired genes into a host to change its phenotype.
  • Bioprocess engineering ensures sterile conditions for large-scale growth of desired microbes or cells to produce products like antibiotics and vaccines.
  • Recombinant DNA technology allows transfer of only specific desirable genes, avoiding unwanted genes common in traditional breeding.
  • For replication, an alien DNA must be linked to an origin of replication, enabling cloning inside the host.
  • Genetic modification involves three steps: identifying the desired gene, introducing it into the host, and maintaining and inheriting it in progeny.
CBSE: Class 12

Key Points: Cloning Vectors

  • Cloning vectors are DNA molecules (plasmids or bacteriophages) that can replicate independently inside host cells and help multiply foreign DNA.
  • An origin of replication (ori) is essential for replication of the vector and controls the copy number of the inserted DNA.
  • Selectable marker genes (e.g., antibiotic resistance genes) help identify and select transformed cells from non-transformants.
  • Cloning sites are unique restriction enzyme recognition sites where foreign DNA is inserted into the vector.
  • Insertional inactivation of marker genes (e.g., β-galactosidase) helps distinguish recombinants from non-recombinants using colour screening.
  • Special vectors like Ti plasmid of Agrobacterium tumifaciens and modified retroviruses are used to transfer genes into plant and animal cells.
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