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Tools of Recombinant DNA Technology - Competent Host (For Transformation with Recombinant DNA)

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Estimated time: 2 minutes
CBSE: Class 12

Key Points: Competent Host (For Transformation with Recombinant DNA)

  • DNA is hydrophilic, so it cannot enter cells easily; bacteria are made competent using Ca²⁺ ions.
  • Cells are treated with cold (ice) and heat shock (42°C) to help the uptake of recombinant DNA.
  • Transformation is the process of introducing recombinant DNA into bacterial cells.
  • Microinjection → DNA is directly injected into the nucleus of animal cells.
  • Biolistics (gene gun) and disarmed pathogens are used to transfer DNA into plant and host cells.
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