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प्रश्न
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
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उत्तर
Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps-
1. Identification and isolation of gene of interest
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule
3. Transfer of this recombinant DNA molecule into suitable host cell
4. Selection of recombinants and non-recombinant cells
5. Expression of gene of interest by culturing recombinant cells under suitable conditions
6. Culturing of cells in bioreactors for large scale production of desired product
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संबंधित प्रश्न
Why must a cell be made ‘competent’ in biotechnology experiments? How does calcium ion help in doing so?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
Why is DNA unable to pass easily through cell membranes without specialized treatment?
In the chemical/temperature method of bacterial transformation, which specific temperature is used for the brief heat shock step?
What is the term used to describe the introduction of recombinant DNA into a bacterial cell?
What type of approach uses a modified pathogen to deliver recombinant DNA into host cells, especially plant systems?
Which divalent cation is used to treat bacterial cells to make them competent for foreign DNA uptake?
