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प्रश्न
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
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उत्तर
Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps-
1. Identification and isolation of gene of interest
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule
3. Transfer of this recombinant DNA molecule into suitable host cell
4. Selection of recombinants and non-recombinant cells
5. Expression of gene of interest by culturing recombinant cells under suitable conditions
6. Culturing of cells in bioreactors for large scale production of desired product
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संबंधित प्रश्न
Why must a cell be made ‘competent’ in biotechnology experiments? How does calcium ion help in doing so?
State the role of “biolistic gun” in biotechnology experiments.
Why should a bacterium be made ‘competent’ ?
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
Why is DNA unable to pass easily through cell membranes without specialized treatment?
In the chemical/temperature method of bacterial transformation, which specific temperature is used for the brief heat shock step?
What is the term used to describe the introduction of recombinant DNA into a bacterial cell?
Which method of introducing recombinant DNA relies on shooting DNA-coated particles into plant cells via particle bombardment?
What type of approach uses a modified pathogen to deliver recombinant DNA into host cells, especially plant systems?
