Advertisements
Advertisements
प्रश्न
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
Advertisements
उत्तर
Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps-
1. Identification and isolation of gene of interest
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule
3. Transfer of this recombinant DNA molecule into suitable host cell
4. Selection of recombinants and non-recombinant cells
5. Expression of gene of interest by culturing recombinant cells under suitable conditions
6. Culturing of cells in bioreactors for large scale production of desired product
APPEARS IN
संबंधित प्रश्न
Why must a cell be made ‘competent’ in biotechnology experiments? How does calcium ion help in doing so?
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
In the chemical/temperature method of bacterial transformation, which specific temperature is used for the brief heat shock step?
Which method introduces recombinant DNA by injecting it directly into the nucleus of animal cells?
Which method of introducing recombinant DNA relies on shooting DNA-coated particles into plant cells via particle bombardment?
What type of approach uses a modified pathogen to deliver recombinant DNA into host cells, especially plant systems?
Which divalent cation is used to treat bacterial cells to make them competent for foreign DNA uptake?
