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प्रश्न
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
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उत्तर
Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps-
1. Identification and isolation of gene of interest
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule
3. Transfer of this recombinant DNA molecule into suitable host cell
4. Selection of recombinants and non-recombinant cells
5. Expression of gene of interest by culturing recombinant cells under suitable conditions
6. Culturing of cells in bioreactors for large scale production of desired product
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संबंधित प्रश्न
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
Why is DNA unable to pass easily through cell membranes without specialized treatment?
In the chemical/temperature method of bacterial transformation, which specific temperature is used for the brief heat shock step?
What is the term used to describe the introduction of recombinant DNA into a bacterial cell?
Which method introduces recombinant DNA by injecting it directly into the nucleus of animal cells?
Which method of introducing recombinant DNA relies on shooting DNA-coated particles into plant cells via particle bombardment?
Which divalent cation is used to treat bacterial cells to make them competent for foreign DNA uptake?
