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प्रश्न
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
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उत्तर
Roles of ‘microinjection’ & ‘gene gun’ in biotechnology
Micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell.
Cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.
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संबंधित प्रश्न
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
Why is DNA unable to pass easily through cell membranes without specialized treatment?
What is the term used to describe the introduction of recombinant DNA into a bacterial cell?
Which method introduces recombinant DNA by injecting it directly into the nucleus of animal cells?
Which method of introducing recombinant DNA relies on shooting DNA-coated particles into plant cells via particle bombardment?
Which divalent cation is used to treat bacterial cells to make them competent for foreign DNA uptake?
