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प्रश्न
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
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उत्तर
In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun. And the last method uses ‘disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host.
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संबंधित प्रश्न
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
In the chemical/temperature method of bacterial transformation, which specific temperature is used for the brief heat shock step?
What is the term used to describe the introduction of recombinant DNA into a bacterial cell?
Which method introduces recombinant DNA by injecting it directly into the nucleus of animal cells?
Which method of introducing recombinant DNA relies on shooting DNA-coated particles into plant cells via particle bombardment?
