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प्रश्न
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
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उत्तर
In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun. And the last method uses ‘disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host.
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संबंधित प्रश्न
Why must a cell be made ‘competent’ in biotechnology experiments? How does calcium ion help in doing so?
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
Why is DNA unable to pass easily through cell membranes without specialized treatment?
In the chemical/temperature method of bacterial transformation, which specific temperature is used for the brief heat shock step?
What is the term used to describe the introduction of recombinant DNA into a bacterial cell?
Which method of introducing recombinant DNA relies on shooting DNA-coated particles into plant cells via particle bombardment?
What type of approach uses a modified pathogen to deliver recombinant DNA into host cells, especially plant systems?
