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Use of ATP and NADPH

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Estimated time: 3 minutes
CBSE: Class 12

Key Points: Use of ATP and NADPH

  • The biosynthetic phase uses ATP and NADPH (from light reactions) to synthesise sugars. Takes place in the stroma of chloroplasts.
  • Called "dark phase", but this is misleading — it does not require darkness but is independent of direct light. It depends on ATP, NADPH, CO₂, and H₂O.
  • After light is removed, biosynthesis continues briefly, then stops. When light returns, it resumes — showing indirect light dependence.
  • Melvin Calvin used radioactive ¹⁴C to discover that the first stable product of CO₂ fixation is 3-phosphoglyceric acid (PGA) — a 3-carbon compound → called the Calvin cycle.
  • C3 pathway - First product of CO₂ fixation = PGA (3-carbon). Found in C3 plants.
  • C4 pathway - First product of CO₂ fixation = Oxaloacetic acid/OAA (4-carbon). Found in C4 plants.
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